Environmental Science & Engineering - www.esemag.com - November 2002
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Teaching bacteria to consume PCBs

A research team from the University of British Columbia and Purdue University is “teaching” microorganisms to break down polychlorinated biphenyls (PCBs) into ecologically safe molecules, a process known as bioremediation.

Scientists have identified one of the key stumbling blocks that prevent microorganisms from decomposing PCBs, a family of persistent hazardous industrial chemicals that is widespread in the environment. They have persisted for decades because decomposers, such as bacteria and fungi, do not consume them very well.

PCBs were manufactured and used in industry for decades for their insulating qualities, but the 1960s and 1970s brought increased awareness of their toxicity to animals and mass poisonings linked to PCB contaminated food. PCBs are no longer manufactured in Canada and the United States, but their persistence makes them a worldwide problem. Many clean-up methods, such as incineration, are ineffective and may generate other toxic compounds such as dioxins.

“The globe’s entire surface is now contaminated with PCBs,” said Jeffrey Bolin, professor of biological sciences and a member of Purdue’s Markey Center for Structural Biology and Cancer Center. “PCB molecules actually look very similar to many organic molecules that certain bacteria eat,” Bolin said. “But there are enough little differences that bacteria can’t quite digest them. The process of digestion requires a long chain of chemical steps, and if the bacteria can’t accomplish one of those steps, the chain is broken and digestion can’t occur,” Bolin said. “What we have done is isolate one of the steps that causes problems for the bacteria, a clog in the biochemical pipeline if you will.”

Bolin and his research partner, Lindsay Eltis, associate professor of microbiology and biochemistry at the University of British Columbia, predict that microorganisms can learn to consume PCBs if properly bred.

“A species will fit itself to a new environment, given many generations to adapt,” said Eltis. “In the case of bacteria, you can get new generations once every few minutes under proper laboratory conditions. We hope to use certain species of bacteria with a slight taste for PCBs and improve this trait through breeding until it’s strong enough to make them consume PCBs as a food source.”

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